How this works
A higher open rate in a small test doesn't always mean a subject line actually won — with too few sends, differences can easily be random chance. This calculator runs a standard statistical significance test on your two variants.

Enter sends and opens for both variants — see whether the difference is real or just noise.
A higher open rate in a small test doesn't always mean a subject line actually won — with too few sends, differences can easily be random chance. This calculator runs a standard statistical significance test on your two variants.
There's no fixed number — it depends on the size of the difference between variants. As a rough guide, tests with fewer than a few hundred sends per variant often can't detect anything but very large differences.
It means that if there were truly no difference between the variants, a gap this large (or larger) would only show up by chance about 5% of the time — not that you're 95% certain which one is better.